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SIX INDEPENDENT VEGETABLE TRIALS · SPAIN 2024

30% less nitrogen. Full-N yield. Six trials out of six.

AGNYTE is a foliar biological for high-value vegetable production. In six independent, replicated trials in lettuce, pepper and cucumber, two 50 g/ha applications integrated with 70% of the standard nitrogen programme matched or exceeded the full-nitrogen control in every trial.

Takes two minutes. We will be in touch to talk through what you grow and how AGNYTE can help.

GEP-compliant · Independent (ANADIAG Ibérica) · Four replications · Three crops

What it means

30%

less applied nitrogen in the trial programme

104–129%

of full-N yield with two AGNYTE applications at 70% N, across all six trials

+49–84%

more yield per kilogram of applied nitrogen vs the full-N programme, across all six trials

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THE HORTICULTURE CHALLENGE

more

nitrogen

is not always

more value

High-value vegetables need rapid, uniform growth, and nitrogen is usually the input growers are least willing to cut. These trials tested exactly that risk: a 30% reduction in applied nitrogen, with AGNYTE added to the programme, against the full standard rate.

What the current data support:

  • Total yield held or improved in every trial. With two applications of AGNYTE at 70% N, total yield ranged from 104% to 129% of the full-nitrogen control across lettuce, pepper and cucumber.

  • Every kilogram of nitrogen worked harder. In Pepper trial 1, yield per kilogram of applied nitrogen rose from 44.2 to 76.5 kg/kg, a 72.8% improvement (p = 0.0001). Nutrient use efficiency indices improved in the AGNYTE-treated plots across the programme.

  • Clean crop safety record. No phytotoxicity and no effects on non-target organisms were recorded in any of the six trials.

Bell Pepper

Results:

Two applications at 70% N: +20.8% total yield vs the full-N control; treatment effect p = 0.03 (ANOVA). Yield per kg applied N +72.8% (p = 0.0001).

Field evidence worth assessing.

Biologicals should not be judged on their claims alone. They should be judged on performance under real field conditions.

Six trials. Three crops. Full-N yield or better in every one.

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Trial 1:

+20.8% (treatment effect p = 0.03)
 (compared to 100% N control)

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Trial 2:

+29.1% yield increase 

(compared to 100% N control)

​

View graphs

Randomised complete block, four replications, small-plot. Lettuce in open field; pepper and cucumber under greenhouse. Full nitrogen rates 103–144 kg N/ha depending on trial; reduced programme at 70% of each. AGNYTEｘ1 andｘ2 foliar applications at 50 g/ha.

HOW AGNYTE WORKS

Biological nitrogen, delivered through the leaf.

Microscopic image of beneficial agricultural microbes used in a microbial consortium.

AGNYTE combines three complementary plant-beneficial bacteria — Azospirillum brasilense, Azotobacter chroococcum and Bacillus megaterium — in a micro-encapsulated foliar formulation designed for survival in the pack and on the leaf.

Once applied, the bacteria colonise plant tissue and work alongside the existing nutrition programme rather than replacing it.  AGYNYTE:

  • Fixes atmospheric nitrogen inside plant tissue

  • Improves availability of phosphorus and potassium

  • Produces phytohormones that support root development and nutrient uptake

Using AGNYTE in Vegetables

Two applications. One existing spray pass each.
Rate
50 g/ha in 200–600 L/ha water, foliar
First application
~10 days after transplant, or 20 days from seed
Second application
15–30 days after the first, depending on cycle length

See How AGNYTE Performs on Your Farm

We'd rather you saw it in your own conditions than took our word for it. Tell us what you grow and where, and we'll send AGNYTE samples for you to run alongside your current programme, including our full technical pack.

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FAQs

  • No. In the trials, AGNYTE was used alongside a reduced nitrogen programme (70% of the standard rate), not instead of nitrogen. It is a nutrient use efficiency tool, not a substitute for sound fertiliser agronomy.

  • Lettuce, bell pepper and cucumber, in six independent GEP-compliant trials run by ANADIAG Ibérica in Sevilla, Spain, in 2024. Two trials per crop, four replications each.

  • 30%. Full-rate programmes ranged from 103 to 144 kg N/ha depending on the trial; the AGNYTE treatments received 70% of that.

  • It varied by trial. All six trials showed a numerical total-yield advantage for two applications of AGNYTE at 70% N over the full-N control. In Pepper trial 1 the treatment effect on total yield was significant (ANOVA p = 0.03), as was the improvement in yield per kilogram of applied nitrogen (p = 0.0001). Across the programme, the most consistently significant results were in nutrient use efficiency indices rather than total yield. Full reports are available on request.

  • For vegetable crops, AGNYTE is typically first applied 10 days after transplant or 20 days from seed. A second application can then be made approximately 15 to 30 days after the first, depending on the length of the growing cycle. 

  • As a foliar spray at 50 g/ha in 200–600 L/ha of water: first application around 10 days after transplant (or 20 days from seed), second 15–30 days later. It can go out with an existing spray pass.

  • AGNYTE is a microbial crop nutrition product designed to improve nutrient delivery and uptake by the plant. AGNYTE contains a consortium of bacteria that fix atmospheric nitrogen, solubilise phosphorus, help mobilise potassium, and increase phytohormone production.

© 2026 by Agnetic Bio Innovations

FARMERS & AGRONOMISTS on AGNYTE's RESULTS

Feedback from the Field

AGNYTE is a Swiss army knife for us because we're getting the stress mitigation factors and the nitrogen aspect of it. And then also you have the phosphorus and potassium solubilisers. And as far as I'm aware, there's really not another product that works on the ratio of nitrogen to potassium.

- Andy J.  (Grower, South Dakota)

AGNYTE is for nitrogen fixation...it's helping out there. As commodity prices tighten up, we need to do everything we can for the farmer... we're going to need to raise every bushel that we can and we're going to need to do it in the most cost-effective way possible.

-Jake F. (Agronomist & Distributor, Michigan)

A lot of guys would always do 100 lbs of urea. Well, we're not really doing that anymore because we just use AGNYTE instead.....they're happy because they don't have to pay, you know, way more money for the urea. They're already making the pass anyway. So, just throw it in the tank.

- Chris (Grower, South Dakota)

 Where I see AGNYTE as a fit is where fertiliser prices are high ...[if a grower] wants to cut back a little bit on fertiliser,  this will help...to move some of those dollars from commercial fertiliser into AGNYTE...Anywhere we had AGNYTE we had a very positive return of at least 3 to 1.

- Troy T. (Co-Op, Colorado)

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